For: E. coli and total coliforms (EPA approved for drinking water compliance monitoring)
Description: Coliscan® C MF is a clear medium containing two chromogenic substrates. One is cleaved by the enzyme galactosidase (produced almost exclusively by coliforms) to produce a non-diffusive pink compound. This causes coliform colonies to become pink/red. The second is cleaved by the enzyme glucuronidase (produced almost exclusively by E. coli) to produce a non-diffusive teal compound. Using the two compounds allows easy visual distinction between general coliforms (pink/red colonies) and E. coli (pink+ teal = blue/purple colonies).
Composition: Contains nutrients, chromogenic substrates and inhibitors.
Procedure: Prepare the medium as instructed. Use standard methodology for product being tested.
Incubation time and temperature:
24-48 hrs at 35°C.
Colors will intensify and colony size will be larger at 36-48 hours.
Interpretation: E. coli colonies will have a blue/purple overall colony color. Other coliforms (Enterobacter, Citrobacter and Klebsiella spp.) are pink to red in color. Proteus, Salmonella and Shigella will generally grow as white colonies. An occasional teal colony is indicative of a non-coliform which has the ability to produce glucuronidase but not galactosidase. A few strains of Salmonella and Shigella have this capability.
Medium shelf life: 1 year (frozen)
E.coli / coliform membrane filter medium 20 mL: Medium for the growth of E. coli and Coliforms with membrane filtration.
Each bottle contains medium for 10 tests.
Discounted to $16.04 per set for orders of 10 or more : Please call (888) EAS-YGEL to place order.
Coliscan® is a registered trademark of Micrology Laboratories